The main purpose of this legislation is to prevent organizations from receiving federal funding if they allow individuals to use single-sex facilities, such as restrooms or locker rooms, that do not align with their biological sex. The bill aims to protect what it describes as "women's spaces" by ensuring that access to these facilities is restricted based on biological sex. Key provisions of the legislation include a clear prohibition on federal funding for any entity that permits access to single-sex facilities for individuals whose gender identity does not match their biological sex.